Anti-DFFB (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O76075
Gene Id	1677
Gene Symbols	DFFB
Protein Name / Synonyms	DFFB (N-term)
Clonality	Polyclonal
Preparation	This DFFB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human DFFB.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.

Specificity

Other Names

DFFB; CAD; DFF2; DFF40; DNA fragmentation factor subunit beta; Caspase-activated deoxyribonuclease; Caspase-activated nuclease; DNA fragmentation factor 40 kDa subunit

NCBI Accession #

NP_004393.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

39110

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; FC: 1:10-50

	Western blot analysis of DFFB Antibody (N-term) (Cat# 102-18414) in 293 cell line lysates (35ug/lane). DFFB (arrow) was detected using the purified Pab.
	DFFB Antibody (N-term) (Cat# 102-18414) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DFFB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	DFFB Antibody (N-term) (Cat# 102-18414) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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