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Anti-DGKA (C-term) Antibody
Catalog #: 102-17812
Rabbit Anti-Human DGKA (C-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P23743 |
| Gene Id | 1606 |
| Gene Symbols | DGKA |
| Protein Name / Synonyms | DGKA (C-term) |
| Clonality | Polyclonal |
| Preparation | This DGKA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 598-628 amino acids from the C-terminal region of human DGKA. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
Upon cell stimulation, the kinase DGKA converts the second messenger diacylglycerol into phophatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity. DGKA is stimulated by calcium and phosphatidylserine, and is phosphorylated by protein kinase C. Tissue expression is in lymphocytes and oligodengroglial cells. DGKA contains 2 zinc-dependent phorbol-ester and DAG binding domains, and 2 EF-hand calcium binding domains.
Specificity
Other Names
DGKA; DAGK; DAGK1; Diacylglycerol kinase alpha; 80 kDa diacylglycerol kinase; Diglyceride kinase alpha
NCBI Accession #
NP_001336.2;NP_958852.1;NP_958853.1;NP_963848.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
82630
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100
 | Western blot analysis of anti-DGKA Antibody (C-term) (Cat# 102-17812) in HL60 cell line lysates (35ug/lane). DGKA (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.