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Anti-DHFR (C-term) Antibody
Catalog #: 102-17312
Rabbit Anti-Mouse DHFR (C-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Mouse |
| Host Species | Rabbit |
| Accession Number | P00375 |
| Gene Id | 13361 |
| Gene Symbols | DHFR |
| Protein Name / Synonyms | DHFR (C-term) |
| Clonality | Polyclonal |
| Preparation | This Mouse DHFR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-164 amino acids from the C-terminal region of mouse DHFR. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.
Specificity
Other Names
Dhfr; Dihydrofolate reductase
NCBI Accession #
NP_034179.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
21606
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100
 | Western blot analysis of DHFR antibody (C-term) (Cat# 102-17312) in mouse liver tissue lysates (35ug/lane). KLF4 (arrow) was detected using the purified Pab. |
 | DHFR Antibody (C-term) (Cat# 102-17312) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DHFR Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | Western blot analysis of DHFR (arrow) using rabbit polyclonal DHFR Antibody (C-term) (Cat# 102-17312). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DHFR gene. |
Antigen Source
MOUSE
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.