Anti-DIAPH3 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9NSV4
Gene Id	81624
Gene Symbols	DIAPH3
Protein Name / Synonyms	DIAPH3, AN, AUNA1, DIA2, DRF3, NSDAN, diap3, mDia2, diaphanous related formin 3
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the diaphanous subfamily of the formin family. Members of this family are involved in actin remodeling and regulate cell movement and adhesion. Mutations in this gene are associated with autosomal dominant auditory neuropathy 1. Multiple transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW

79kDa/98kDa/127-136kDa

Observed MW

140kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 630-849 of human DIAPH3 (NP_112194.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using DIAPH3 antibody (144-600531) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - DIAPH3 Polyclonal Antibody (144-600531)

Expiration:

12 months from the date of shipment when stored properly.

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