Anti-DIO3 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P55073
Gene Id	1735
Gene Symbols	DIO3
Protein Name / Synonyms	DIO3 (C-term)
Clonality	Polyclonal
Preparation	This DIO3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-278 amino acids from the C-terminal region of human DIO3.
Concentration (lot specific)	0.500 mg/ml
Recommended Applications	Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

DIO3 belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. This enzyme is highly expressed in the pregnant uterus, placenta, fetal and neonatal tissues, suggesting that it plays an essential role in the regulation of thyroid hormone inactivation during embryological development. This protein contains a selenocysteine (Sec) residue, which is essential for efficient enzyme activity. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal.

Specificity

Other Names

DIO3; ITDI3; TXDI3; Type III iodothyronine deiodinase; 5DIII; DIOIII; Type 3 DI; Type-III 5'-deiodinase

NCBI Accession #

NP_001353.4

Other Accession #

NP_001353

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

31451

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of DIO3 Antibody (C-term) (Cat# 102-18301) in HepG2 cell line lysates (35ug/lane). DIO3 (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with DIO3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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