Synonyms
DMC1H antibody, HsLim15 antibody, LIM15 antibody, MGC150472 antibody, MGC150473 antibody, dJ199H16.1 antibody, DMC1 antibody, meiotic recombination protein DMC1/LIM15 homolog antibody, DMC1 homologue antibody, "disrupted meiotic cDNA1, yeast, homolog of antibody", "DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast) antibody"
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
38 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 206 of DMC1 (Uniprot ID# Q14565)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

DMC1 antibody [N1C1] detects DMC1 protein at nucleolus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
DMC1 antibody [N1C1] (107-10363) dilution: 1:500.

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using DMC1(107-10363) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: Molt-4
10% SDS PAGE
107-10363 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.