RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

www.RayBiotech.com | info@raybiotech.com

770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-DMC1 Antibody

Catalog #: 107-10363

DMC1 antibody [N1C1], 0.9 mg/ml (100 ul)

Product Description

Specifications

Size100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman
Host SpeciesRabbit
Gene Id
11144
Protein Name / Synonyms
DMC1
Concentration (lot specific)
0.9 mg/ml
Recommended ApplicationsIHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping TypeBlue ice
Storage-20°C

Description

Synonyms

DMC1H antibody, HsLim15 antibody, LIM15 antibody, MGC150472 antibody, MGC150473 antibody, dJ199H16.1 antibody, DMC1 antibody, meiotic recombination protein DMC1/LIM15 homolog antibody, DMC1 homologue antibody, "disrupted meiotic cDNA1, yeast, homolog of antibody", "DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast) antibody"

Specificity

Species Reactivity
Human

Format

Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
38 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 206 of DMC1 (Uniprot ID# Q14565)

Applications

Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
DMC1 antibody [N1C1] detects DMC1 protein at nucleolus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
DMC1 antibody [N1C1] (107-10363) dilution: 1:500.
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using DMC1(107-10363) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: Molt-4
10% SDS PAGE
107-10363 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.