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Anti-DNA ligase III Antibody
Catalog #: 107-10370
DNA ligase III antibody, 1.01 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 3980 |
| Protein Name / Synonyms | DNA ligase III |
| Concentration (lot specific) | 1.01 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
LIG2 antibody, LIG3 antibody, ligase II, DNA, ATP-dependent antibody, polydeoxyribonucleotide synthase [ATP] 3 antibody, DNA ligase 3 antibody, ligase III, DNA, ATP-dependent antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
113 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 116 and 433 of DNA ligase 3 (Uniprot ID# P49916)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
DNA ligase III antibody detects LIG3 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LIG3 protein stained by DNA ligase III antibody (107-10370) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LIG3 protein stained by DNA ligase III antibody (107-10370) diluted at 1:500.
Blue: Hoechst 33342 staining.
DNA ligase III antibody detects DNA ligase III protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody (107-10370) diluted by 1:1000.
Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using DNA ligase 3(107-10370) antibody at 1:100 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.