DNA ligase III antibody [C2C3], C-Terminus, 0.31 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 3980 |
| Protein Name / Synonyms | DNA ligase III, C-term |
| Concentration (lot specific) | 0.31 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections) |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
LIG2 antibody, LIG3 antibody, ligase II, DNA, ATP-dependent antibody, polydeoxyribonucleotide synthase [ATP] 3 antibody, DNA ligase 3 antibody, ligase III, DNA, ATP-dependent antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
113 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 765 and 1009 of DNA ligase 3 (Uniprot ID# P49916)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections)
Application Notes
Suggested starting dilutions are as follows: IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of paraformaldehyde-fixed A431, using DNA ligase 3(107-10372) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded H441 Xenograft, using DNA ligase 3(107-10372) antibody at 1:100 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.