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Anti-DNA polymerase gamma Antibody
Catalog #: 107-10221
DNA polymerase gamma antibody, 0.67 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Rat |
| Host Species | Rabbit |
| Gene Id | 5428 |
| Protein Name / Synonyms | DNA polymerase gamma |
| Concentration (lot specific) | 0.67 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
FLJ27114 antibody, MDP1 antibody, MIRAS antibody, MTDPS4A antibody, MTDPS4B antibody, PEO antibody, POLG1 antibody, POLGA antibody, SANDO antibody, SCAE antibody, POLG antibody, "PolG, catalytic subunit antibody", DNA polymerase subunit gamma-1 antibody, mitochondrial DNA polymerase catalytic subunit antibody, PolG-alpha antibody, "polymerase (DNA directed), gamma antibody"
Specificity
Species Reactivity
Human, Rat
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
140 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 779 and 1231 of DNA polymerase gamma (Uniprot ID# P54098)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed HeLa, using DNA polymerase gamma(107-10221) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded Skeletal Muscle , using DNA polymerase gamma(107-10221) antibody(10 µg/ml).
Sample (30 ug of whole cell lysate)
A: MCF-7
5% SDS PAGE
107-10221 diluted at 1:1000
A: MCF-7
5% SDS PAGE
107-10221 diluted at 1:1000
DNA polymerase gamma antibody detects POLG protein by Western blot analysis.
A. 50 µg Rat testis lysate/extract
5% SDS-PAGE
DNA polymerase gamma antibody (107-10221) dilution: 1:1000
A. 50 µg Rat testis lysate/extract
5% SDS-PAGE
DNA polymerase gamma antibody (107-10221) dilution: 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.