Synonyms
HSJ1 antibody, HSPF3 antibody, DNAJB2 antibody, dnaJ protein homolog 1 antibody, heat shock 40 kDa protein 3 antibody, heat shock protein J1 antibody, dnaJ homolog subfamily B member 2 antibody, heat shock protein, neuronal DNAJ-like 1 antibody, DnaJ (Hsp40) homolog, subfamily B, member 2 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
36 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 238 of DNAJB2 (Uniprot ID# P25686)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

DNAJB2 antibody detects DNAJB2 protein at cytosol on Ovarian Carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded Ovarian Carcinoma.
DNAJB2 antibody (107-10453) dilution: 1:500.

Sample (30 ug of whole cell lysate)
A: A431
10% SDS PAGE
DNAJB2 antibody
107-10453 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.