Anti-Dnmt3b Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9UBC3
Gene Id	1789
Gene Symbols	DNMT3B
Protein Name / Synonyms	Dnmt3b
Clonality	Polyclonal
Preparation	This Dnmt3b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-417 amino acids from human Dnmt3b.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. Dnmt3b is a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF)syndrome.

Specificity

Other Names

DNMT3B; DNA (cytosine-5)-methyltransferase 3B; DNA methyltransferase HsaIIIB

NCBI Accession #

NP_001193984.1;NP_001193985.1;NP_008823.1;NP_787044.1;NP_787045.1;NP_787046.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

95751

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	Western blot analysis of anti-Dnmt3b Pab (Cat# 102-10628) in T47-D cell lysate. Dnmt3b (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
	Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with Dnmt3b antibody (Cat# 102-10628), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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