Anti-DOK4 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q8TEW6
Gene Id	55715
Gene Symbols	DOK4
Protein Name / Synonyms	DOK4 (C-term)
Clonality	Polyclonal
Preparation	This DOK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-258 amino acids from the C-terminal region of human DOK4.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

Specificity

Predicted Reactivity

Mouse

Other Names

DOK4; Docking protein 4; Downstream of tyrosine kinase 4; Insulin receptor substrate 5

NCBI Accession #

NP_060580.2

Other Accession #

Q99KE3; NP_060580

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

37028

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of DOK4 (C-term) (arrow) using DOK4 Antibody (C-term) (Cat# 102-17564). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DOK4 gene (Lane 2) (Origene Technologies).
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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