RayBiotech

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Anti-Dopamine beta hydroxylase Antibody

Catalog #: 107-10273

Dopamine beta hydroxylase antibody, 1 mg/ml (100 ul)

Product Description

Specifications

Size100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Gene Id
1621
Protein Name / Synonyms
Dopamine beta hydroxylase
Concentration (lot specific)
1 mg/ml
Recommended ApplicationsICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Research AreaNeuroscience, Hydroxylation, Post-Translational Modifications
Shipping TypeBlue ice
Storage-20°C

Description

Synonyms

DBM antibody, DBH antibody, dopamine beta-hydroxylase antibody, dopamine beta-hydroxylase (dopamine beta-monooxygenase) antibody

Specificity

Species Reactivity
Human, Rat, Mouse

Format

Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
69 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 8 and 320 of Dopamine beta Hydroxylase (Uniprot ID# P09172)

Applications

Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Dopamine beta hydroxylase antibody detects DBH protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
Dopamine beta hydroxylase antibody (107-10273) dilution: 1:500.
Dopamine beta hydroxylase antibody detects DBH protein at cytosol on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Dopamine beta hydroxylase antibody (107-10273) dilution: 1:500.
Dopamine beta hydroxylase antibody detects DBH protein at cytosol on RT2 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded RT2 xenograft.
Dopamine beta hydroxylase antibody (107-10273) dilution: 1:500.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Dopamine beta-Hydroxylase(107-10273) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate)
A: Hep G2
7.5% SDS PAGE
107-10273 diluted at 1:5000

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.