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Anti-Doublesex Antibody, Biotinylated
Catalog #: 130-00020B-50
Mouse anti-Doublesex Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Insect |
| Host Species | Mouse |
| Accession Number | AAA17840 |
| Clonality | Monoclonal |
| Immunogen | Immunogen was recombinant protein derived from Drosophila Doublesex (aa35-93). This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography. |
| Isotype | IgG1 |
| Recommended Applications | ELISA (recommended work dilution= 1: 1, 000-5, 000), Western Blotting (recommended work dilution= 1: 1, 000-2, 000) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
The doublesex (dsx) gene regulates somatic sexual differentiation in both sexes in D. melanogaster. Two functional products are encoded by dsx: one product is expressed in females and represses male differentiation, and the other is expressed in males and represses female differentiation. The protein functions are needed to further investigate.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
This antibody was selected for its ability to detect Drosophila Doublesex (aa35-93). In ELISA and Western Blot, this antibody showed specifically binding to recombinant protein from Drosophila Doublesex and there was no cross-reactivity with tested proteins from Drosophila Doublesex.
References
- Baker, B. S., and Belote, J. M. (1983). Sex determination and dosage compensation in Drosophila melanogasfer. Annu. Rev. Genet. 77,345-393.
- Oliver, B., Perrimon, N.. and Mahowald, A. l? (1988). Genetic evidence that the saris fille locus is involved in Drosophila sex determination.Genetics 720, 159-171.
Expiration:
12 months from the date of shipment when stored properly.