Anti-DPYD (N-term) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	1806
Protein Name / Synonyms	DPYD, N-term
Concentration (lot specific)	0.59 mg/ml
Recommended Applications	IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

DHP antibody, DHPDHASE antibody, DPD antibody, MGC132008 antibody, MGC70799 antibody, DPYD antibody, dihydrouracil dehydrogenase antibody, dihydropyrimidine dehydrogenase [NADP+] antibody, dihydrothymine dehydrogenase antibody, dihydropyrimidine dehydrogenase antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

111 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 174 of (Uniprot ID# Q12882)

Applications

Applications Tested

IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

DPYD antibody [N1N2], N-term detects DPYD protein at cytoplasm on H358 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H358 xenograft.
DPYD antibody [N1N2], N-term (107-11086) dilution: 1:500.

Sample (30 ug of whole cell lysate)
A: Raji
B: K562
C: NCI-H929
7.5% SDS PAGE
107-11086 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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