Anti-DTL (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q9NZJ0
Gene Id	51514
Gene Symbols	DTL
Protein Name / Synonyms	DTL (Center)
Clonality	Polyclonal
Preparation	This DTL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 229-256 amino acids from the Central region of human DTL.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis.

Specificity

Other Names

DTL; CDT2; CDW1; DCAF2; L2DTL; RAMP; Denticleless protein homolog; DDB1- and CUL4-associated factor 2; Lethal(2) denticleless protein homolog; Retinoic acid-regulated nuclear matrix-associated protein

NCBI Accession #

NP_057532.2

Other Accession #

Q3TLR7; NP_057532.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

79468

Recommended Dilutions

WB: 1:1000; FC: 1:10-50

	DTL Antibody (Center) (Cat# 102-10880) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the DTL antibody detected the DTL protein (arrow).
	DTL Antibody (Center) (Cat# 102-10880) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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