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Anti-E Cadherin (CDH1) (N-term) Antibody
Catalog #: 102-12650
Rabbit Anti-Human E Cadherin (CDH1) (N-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P12830 |
| Gene Id | 999 |
| Gene Symbols | CDH1 |
| Protein Name / Synonyms | E Cadherin (CDH1) (N-term) |
| Clonality | Polyclonal |
| Preparation | This E Cadherin (CDH1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-189 amino acids from the N-terminal region of human E Cadherin (CDH1). |
| Concentration (lot specific) | 2.000 mg/ml |
| Recommended Applications | Western Blotting, FC |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
CDH1 is a classical cadherin from the cadherin superfamily. This protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization.
Specificity
Other Names
CDH1; CDHE; UVO; E-Cad/CTF3; Cadherin-1; CAM 120/80; Epithelial cadherin; Uvomorulin; CD_antigen=CD324; E-Cad/CTF1; E-Cad/CTF2
NCBI Accession #
NP_004351.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
97456
Recommended Dilutions
WB: 1:1000; FC: 1:10-50
 | Western blot analysis of CDH1 Antibody (N-term) (Cat# 102-12650) in 293 cell line lysates (35ug/lane). CDH1 (arrow) was detected using the purified Pab. |
 | Western blot analysis of CDH1 (arrow) using rabbit polyclonal CDH1 Antibody (N-term) (Cat# 102-12650).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH1 gene (Lane 2) (Origene Technologies). |
 | Flow cytometric analysis of NCI-H292 cells using E Cadherin (CDH1) Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.