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Anti-E-tag Antibody, Biotinylated
Catalog #: RB-10-0004B-50
Mouse anti-E-tag Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Specificity | E-Tag antibody detects fusion recombinant antibodies derived from a variety of sources at various concentrations. No cross reactivity with other-tags-fusion protein was found in ELISA or Western Blot. |
| Host Species | Mouse |
| Clonality | Monoclonal |
| Immunogen | A synthetic peptide corresponding to residues GAPVPYPDPLEPR coupled to KLH. This antibody was produced from a hybridoma resulting from the fusion of E-tags with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G affinity chromatography. |
| Isotype | IgG1 |
| Recommended Applications | ELISA (recommended work dilution= 1:1000-5000), Western Blotting (recommended work dilution= 1:1, 000 |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Plasmid vectors are commonly used for the expression of hybrid fusion proteins, encoding a protein of interest fused to an epitope tag. Antibodies specific for such fusion protein tags are useful for monitoring expression of the protein of interest, both in cells and in Western blot analysis. Such epitope tags in common use include Myc, GST, His, HA, Flag and HN, etc. E-tag is one of novel series of epitope tags. It commonly applies to detect recombinant antibody with the tag. Anti-E-Tag antibody is suitable for purification, detection and immunoprecipitation of recombinant fusion proteins containing the respective E-Tag.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
E-Tag antibody detects fusion recombinant antibodies derived from a variety of sources at various concentrations. No cross reactivity with other-tags-fusion protein was found in ELISA or Western Blot.
Expiration:
12 months from the date of shipment when stored properly.