RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

www.RayBiotech.com | info@raybiotech.com

770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-E-tag Antibody

Catalog #: RB-10-0004

Mouse Anti-E-tag Antibody

Product Description

Specifications

Size20 µg, 100 µg, 200 µg, 500 µg
Estimated Lead Time1-2 business days
Specificity
E-Tag antibody detects fusion recombinant antibodies derived from a variety of sources at various concentrations. No cross reactivity with other-tags-fusion protein was found in ELISA or Western Blot.
ClonalityMonoclonal
Clone3H6-F5
Immunogen
A synthetic peptide corresponding to residues GAPVPYPDPLEPR coupled to KLH. This antibody was produced from a hybridoma resulting from the fusion of E-tags with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G affinity chromatography.
Recommended ApplicationsELISA (recommended work dilution= 1:1000-5000), Western Blotting (recommended work dilution= 1:1, 000
Reconstitution
Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml.
Shipping TypeAmbient temperature
Storage-20°C

Description

Description

Plasmid vectors are commonly used for the expression of hybrid fusion proteins, encoding a protein of interest fused to an epitope tag. Antibodies specific for such fusion protein tags are useful for monitoring expression of the protein of interest, both in cells and in Western blot analysis. Such epitope tags in common use include Myc, GST, His, HA, Flag and HN, etc. E-tag is one of novel series of epitope tags. It commonly applies to detect recombinant antibody with the tag. Anti-E-Tag antibody is suitable for purification, detection and immunoprecipitation of recombinant fusion proteins containing the respective E-Tag.

Specificity

E-Tag antibody detects fusion recombinant antibodies derived from a variety of sources at various concentrations. No cross reactivity with other-tags-fusion protein was found in ELISA or Western Blot.

Storage

Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can also be aliquoted and stored frozen at -20°C to -80°C in a manual defrost freezer for 6 months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for 30 days at 4°C. Please avoid freeze-thaw cycles, as this will lower the activity of the antibody.
Expiration:
12 months from the date of shipment when stored properly.