Mouse Anti-E2F-1 Antibody, 100 µl
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Mouse |
| Accession Number | Q01094 |
| Gene Id | 1869 |
| Gene Symbols | E2F1 |
| Protein Name / Synonyms | DmelCG63 |
| Clonality | Monoclonal |
| Recommended Applications | Western Blotting, ICC, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis.
Other Information
Antigen Type
Recombinant Protein
Images
WB (1:500)
Western blot detection of E2F-1 in C6,Raw264.7 and Hela cell lysates using E2F-1 mouse mAb (1:500 diluted).Predicted band size:70KDa.Observed band size:70KDa.
ICC (1:100)
Immunofluorescent analysis of Hela cells fixed with 4% Paraformaldehyde and using anti-E2F-1 mouse mAb (dilution 1:100). DAPI was used to stain nucleus(blue).
IP (1:500)
Immunoprecipitation analysis of Hela cell lysates using E2F-1 mouse mAb.
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.