Anti-E2F5 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q15329
Gene Id	1875
Gene Symbols	E2F5
Protein Name / Synonyms	E2F5 (N-term)
Clonality	Polyclonal
Preparation	This E2F5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-93 amino acids from the N-terminal region of human E2F5.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

E2F5 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB.

Specificity

Predicted Reactivity

Mouse, Rat

Other Names

E2F5; Transcription factor E2F5

NCBI Accession #

NP_001077057.1;NP_001077058.1;NP_001942.2

Other Accession #

Q62814; Q61502; Q8R0K9; Q16254

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

37610

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Western blot analysis of E2F5 Antibody (N-term) (Cat# 102-18112) in HepG2 cell line lysates (35ug/lane). E2F5 (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with E2F5 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	E2F5 Antibody (N-term) (Cat# 102-18112) FC analysis of ZR-75-1 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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