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Anti-ECSIT (C-term) Antibody
Catalog #: 102-12690
Rabbit Anti-Human ECSIT (C-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q9BQ95 |
| Gene Id | 51295 |
| Gene Symbols | ECSIT |
| Protein Name / Synonyms | ECSIT (C-term) |
| Clonality | Polyclonal |
| Preparation | This ECSIT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 396-425 amino acids from the C-terminal region of human ECSIT. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
Adapter protein of the Toll-like and IL-1 receptor signaling pathway that is involved in the activation of NF-kappa-B via MAP3K1. Promotes proteolytic activation of MAP3K1. Involved in the BMP signaling pathway. Required for normal embryonic development (By similarity). Required for efficient assembly of mitochondrial NADH:ubiquinone oxidoreductase.
Specificity
Other Names
ECSIT; Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial; Protein SITPEC
NCBI Accession #
NP_001135936.1;NP_001135937.1;NP_057665.2
Other Accession #
NP_001135936.1; NP_057665.2
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
49148
Recommended Dilutions
WB: 1:1000; IF: 1:10-50
 | ECSIT Antibody (C-term) (Cat# 102-12690) western blot analysis in Jurkat,ZR-75-1 cell line lysates (35ug/lane).This demonstrates the ECSIT antibody detected the ECSIT protein (arrow). |
 | Fluorescent confocal image of Hela cell stained with ECSIT Antibody (C-term)(Cat# 102-12690).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ECSIT primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ECSIT immunoreactivity is localized to Cytoplasm and Nucleus significantly. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.