Rabbit Anti-Human EEF1A1 (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P68104 |
| Gene Id | 1915 |
| Gene Symbols | EEF1A1 |
| Protein Name / Synonyms | EEF1A1 (C-term) |
| Clonality | Polyclonal |
| Preparation | This EEF1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 361-389 amino acids from the C-terminal region of human EEF1A1. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, FC, IF, IHC-P |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
EEF1A1 is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome.
Specificity
Predicted Reactivity
Mouse, Rat, Zebrafish, Bovine, Chicken, Hamster, Rabbit, Xenopus
Other Names
EEF1A1; EEF1A; EF1A; LENG7; Elongation factor 1-alpha 1; Elongation factor Tu; Eukaryotic elongation factor 1 A-1; Leukocyte receptor cluster member 7
NCBI Accession #
NP_001393.1
Other Accession #
Q92005; Q90835; Q5VTE0; P62632; Q71V39; P62631; Q05639; Q32PH8; P62630; P68105; P10126; P62629; P68103; P13549
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
50141
Recommended Dilutions
WB: 1:1000; FC: 1:10-50; IF: 1:10-50; IHC: 1:10-50
 | Western blot analysis of EEF1A1 Antibody (C-term) (Cat# 102-16841) in MCF-7 cell line lysates (35ug/lane). EEF1A1 (arrow) was detected using the purified Pab. |
 | Flow cytometric analysis of NCI-H292 cells using EEF1A1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Confocal immunofluorescent analysis of EEF1A1 Antibody (C-term)(Cat# 102-16841) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). |
 | EEF1A1 Antibody (C-term) (102-16841)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EEF1A1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.
