EEF2K Polyclonal Antibody
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | O00418 |
| Gene Id | 29904 |
| Gene Symbols | EEF2K |
| Protein Name / Synonyms | EEF2K, HSU93850, eEF-2K, eukaryotic elongation factor 2 kinase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment.
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
82kDa
Observed MW
110kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 406-725 of human EEF2K (NP_037434.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using EEF2K antibody (144-05404) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 40s.![Western blot - [KO Validated] EEF2K Polyclonal Antibody (144-05404)](https://cdn.sanity.io/images/g51potcz/production/779714f85a3087d2b79a49abbf66bb1f4bdf5ec0-398x350.jpg)
Western blot analysis of extracts from normal (control) and EEF2K knockout (KO) HeLa cells, using EEF2K antibody (144-05404) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.![Western blot - [KO Validated] EEF2K Polyclonal Antibody (144-05404)](https://cdn.sanity.io/images/g51potcz/production/4d7b04e0d97967000f5b99b3cfc1ae4485281591-367x550.jpg)
Immunofluorescence analysis of A-549 cells using EEF2K antibody (144-05404). Blue: DAPI for nuclear staining.![Immunofluorescence - [KO Validated] EEF2K Polyclonal Antibody (144-05404)](https://www.raybiotech.com/files/images/Antibodies/144/144-05404/3.jpg)
Expiration:
12 months from the date of shipment when stored properly.