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ISO 13485:2016 cGMP
Anti-EGF Antibody
Catalog #: DS-PB-00693
Rabbit Anti-Human EGF
Product Description
Specifications
| Size | 0.1 mg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Specificity | This product is specific for human EGF (Epidermal Growth Factor), a potent stimulator and regulator of the proliferation of epidermal and epithelial cells, which is expressed by a variety of cells.<br > The effects of EGF are initiated through tyrosine kinase activity, following the binding of EGF to the extracellular domain of the 170kDa EGF receptor (EGFR), which also binds other EGF-like ligands with high affinity, including TGF-α, VGF (vaccinia virus growth factor) and Betacellulin, produced by certain tumour cells. Receptor activation through EGF binding triggers several signal transduction pathways, including the JAK/STAT and P13K/AKT pathways. |
| Host Species | Rabbit |
| Protein Name / Synonyms | EGF |
| Preparation | Antisera to human EGF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography. |
| Concentration (lot specific) | IgG concentration 1.0mg/ml after reconstitution |
| Recommended Applications | ELISA (dilution: 0.1, 0.2ug/ml), Functional Assays, Immunohistology - Paraffin, Western Blotting |
| Reconstitution | Reconstitute with 0.1ml distilled water.For long term storage the addition of 0.09% sodium azide is recommended.N.B. For functional studies do not add azide. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Storage/Stability
Prior to reconstitution store at +4 °C.
After reconstitution store at -20 °C.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.
Format
Supplied as:
Purified IgG - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 0.1ml distilled water.
For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add azide.
For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add azide.
Format Type:
Purified
Preparation
Antisera to human EGF were raised by repeated immunisations of rabbits with highly purified antigen.
Purified IgG prepared by affinity chromatography.
Immunogen:
Recombinant human EGF
Preservative:
None present.
Expiration:
12 months from the date of shipment when stored properly.