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Anti-EGFR Antibody
Catalog #: 144-11577
EGFR Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P00533 |
| Gene Id | 1956 |
| Gene Symbols | EGFR |
| Protein Name / Synonyms | EGFR, ERBB, ERBB1, HER1, NISBD2, PIG61, mENA, epidermal growth factor receptor |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Research Area | Cardiovascular Disease, Cancer, Inflammation, HER/ErbB Signaling, Tyrosine Kinase Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
Format
Calculated MW
44kDa/69kDa/77kDa/134kDa
Observed MW
170kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1021-1210 of human EGFR (NP_005219.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using EGFR antibody (144-011577) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat liver using EGFR antibody (144-011577) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using EGFR antibody (144-011577) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using EGFR antibody (144-011577) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using EGFR antibody (144-011577) at dilution of 1:150 (40x lens).
Immunofluorescence analysis of HeLa cells using EGFR antibody (144-011577) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using EGFR antibody (144-011577) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC12 cells using EGFR antibody (144-011577) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug EGFR antibody (144-011577). Western blot was performed from the immunoprecipitate using EGFR antibody (144-011577) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.