Anti-EGFR Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P00533
Gene Id	1956
Gene Symbols	EGFR
Protein Name / Synonyms	EGFR, ERBB, ERBB1, HER1, NISBD2, PIG61, mENA, epidermal growth factor receptor
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Research Area	Cardiovascular Disease, Cancer, Inflammation, HER/ErbB Signaling, Tyrosine Kinase Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW

44kDa/69kDa/77kDa/134kDa

Observed MW

180kDa

Immunogen

A phospho specific peptide corresponding to residues surrounding Y1045 of human EGFR

Modification

Phospho Y1045

Storage

-20°C

Images

Western blot analysis of extracts of A-431 cells, using Phospho-EGFR-Y1045 antibody (144-61535) at 1:1000 dilution or EGFR antibody (144-11351). A-431 cells were treated by EGF (100 ng/ml) at 37? for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - Phospho-EGFR-Y1045 pAb (144-61535)

Expiration:

12 months from the date of shipment when stored properly.

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