EIF1AY Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O14602 |
| Gene Id | 9086 |
| Gene Symbols | EIF1AY |
| Protein Name / Synonyms | EIF1AY, eIF-4C, eukaryotic translation initiation factor 1A, Y-linked |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene is located on the non-recombining region of the Y chromosome. It encodes a protein related to eukaryotic translation initiation factor 1A (EIF1A), which may function in stabilizing the binding of the initiator Met-tRNA to 40S ribosomal subunits. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:200 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
16kDa
Observed MW
18kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-144 of human EIF1AY (NP_004672.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using EIF1AY antibody (144-04270) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat heart using EIF1AY antibody (144-04270) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using EIF1AY antibody (144-04270) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using EIF1AY antibody (144-04270) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using EIF1AY antibody (144-04270) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.