Anti-EIF2A Antibody

Product Description

Specifications

Size	200 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Mouse
Accession Number	P05198
Gene Id	1965
Gene Symbols	EIF2S1
Protein Name / Synonyms	Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, eIF-2A, eIF-2alpha, EIF2S1, EIF2A
Clonality	Monoclonal
Clone	1763CT102.71.17
Isotype	Kappa, IgG2b
Recommended Applications	Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Other Information

Antigen Type

Recombinant Protein

Format

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

Calculated Molecular Weight (Da)

36112

Antigen Source

Human

Images

WB (1:2000)

All lanes : Anti-EIF2A Antibody at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: PC-12 whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lane 5: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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