Anti-EIF2B beta Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	8892
Protein Name / Synonyms	EIF2B beta
Concentration (lot specific)	0.43 mg/ml
Recommended Applications	IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

EIF-2Bbeta antibody, EIF2B antibody, EIF2B2 antibody, S20I15 antibody, translation initiation factor eIF-2B subunit beta antibody, eIF-2B GDP-GTP exchange factor subunit beta antibody, S20III15 antibody, "eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa antibody"

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

39 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 88 and 323 of EIF2B beta (Uniprot ID# P49770)

Applications

Applications Tested

IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded Colon ca, using EIF2B beta(107-11080) antibody at 1:250 dilution.

Sample (30 ug of whole cell lysate)
A: A431
10% SDS PAGE
107-11080 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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