Anti-EIF2B1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q14232
Gene Id	1967
Gene Symbols	EIF2B1
Protein Name / Synonyms	EIF2B1, EIF2B, EIF2BA, translation initiation factor eIF-2B subunit alpha
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

24kDa/33kDa

Observed MW

34kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-305 of human EIF2B1 (NP_001405.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using EIF2B1 antibody (144-07892) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - EIF2B1 Polyclonal Antibody (144-07892)

Immunofluorescence analysis of U2OS cells using EIF2B1 antibody (144-07892) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - EIF2B1 Polyclonal Antibody (144-07892)

Expiration:

12 months from the date of shipment when stored properly.

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