3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-EIF3C Antibody
Catalog #: 144-07022
EIF3C Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q99613 |
| Gene Id | 8663 |
| Gene Symbols | EIF3C |
| Protein Name / Synonyms | EIF3C, EIF3CL, EIF3S8, eIF3-p110, eukaryotic translation initiation factor 3 subunit C |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:100
Format
Calculated MW
104kDa/105kDa
Observed MW
105kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 694-913 of human EIF3C (NP_003743.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using EIF3C antibody (144-07022) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat brain using EIF3C Antibody (144-07022) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse intestine using EIF3C Antibody (144-07022) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using EIF3C Antibody (144-07022) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using EIF3C antibody (144-07022). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.