Anti-EIF3E (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Zebrafish
Host Species	Rabbit
Accession Number	P60228
Gene Id	3646
Gene Symbols	EIF3E
Protein Name / Synonyms	EIF3E(Center)
Clonality	Polyclonal
Preparation	This EIF3E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 248-276 amino acids from the Central region of human EIF3E.
Concentration (lot specific)	0.500 mg/ml
Recommended Applications	Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine, Chicken, Monkey, Xenopus

Other Names

EIF3E; EIF3S6; INT6; Eukaryotic translation initiation factor 3 subunit E; Eukaryotic translation initiation factor 3 subunit 6; Viral integration site protein INT-6 homolog; eIF-3 p48

NCBI Accession #

NP_001559.1

Other Accession #

Q641X8; P60229; Q4R6G8; Q5ZLA5; Q3T102; Q3B8M3; Q1LUA8; Q05AY2; Q6DRI1; NP_001559.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

52221

Recommended Dilutions

WB: 1:1000

	(DANRE) eif3eb Antibody (Center) (Cat# 102-14266) western blot analysis in zebra fish brain tissue lysates (35ug/lane).This demonstrates the (DANRE) eif3eb antibody detected the (DANRE) eif3eb protein (arrow).
	EIF3E Antibody (Center)(Cat# 102-14266) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the EIF3E antibody detected the EIF3E protein (arrow).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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