Anti-EIF3G (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O75821
Gene Id	8666
Gene Symbols	EIF3G
Protein Name / Synonyms	EIF3G (Center)
Clonality	Polyclonal
Preparation	This EIF3G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209-238 amino acids from the Central region of human EIF3G.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit can bind 18S rRNA.

Specificity

Predicted Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus

Other Names

EIF3G; EIF3S4; Eukaryotic translation initiation factor 3 subunit G; Eukaryotic translation initiation factor 3 RNA-binding subunit; Eukaryotic translation initiation factor 3 subunit 4; eIF-3-delta; eIF3 p42; eIF3 p44

NCBI Accession #

NP_003746.2

Other Accession #

Q5RK09; Q9Z1D1; Q6DRC4; Q3ZC12; Q641B2; Q6DJI8; NP_003746.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

35611

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	EIF3G Antibody (Center) (Cat# 102-16471) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the EIF3G antibody detected the EIF3G protein (arrow).
	EIF3G Antibody (Center) (Cat# 102-16471) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EIF3G Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
	EIF3G Antibody (Center) (Cat# 102-16471) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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