Rabbit Anti-Human EIF4A1 (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P60842 |
| Gene Id | 1973 |
| Gene Symbols | EIF4A1 |
| Protein Name / Synonyms | EIF4A1 (N-term) |
| Clonality | Polyclonal |
| Preparation | This EIF4A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human EIF4A1. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
EIF4A1 is an ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
Specificity
Predicted Reactivity
Mouse, Bovine, Monkey
Other Names
EIF4A1; DDX2A; EIF4A; Eukaryotic initiation factor 4A-I; ATP-dependent RNA helicase eIF4A-1
NCBI Accession #
NP_001191439.1;NP_001407.1
Other Accession #
P60843; Q4R8K5; Q3SZ54
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
46154
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50
 | Western blot analysis of anti-EIF4A1 Pab (Cat# 102-17220) in K562 cell line lysates (35ug/lane). EIF4A1(arrow) was detected using the purified Pab. |
 | Western blot analysis of EIF4A1 (arrow) using rabbit polyclonal EIF4A1 Antibody (N-term) (Cat# 102-17220). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF4A1 gene (Lane 2) (Origene Technologies). |
 | Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EIF4A1 antibody (N-term) (Cat# 102-17220), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.
