Anti-EIF4B (S422) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P23588
Gene Id	1975
Gene Symbols	EIF4B
Protein Name / Synonyms	EIF4B (S422)
Clonality	Polyclonal
Preparation	This EIF4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 400-429 amino acids from human EIF4B.
Concentration (lot specific)	0.380 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with EIF4-F and EIF4-A by binding near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. It also promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F

Specificity

Other Names

EIF4B; Eukaryotic translation initiation factor 4B

NCBI Accession #

NP_001408.2

Other Accession #

Q8WYK5

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

69151

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	Western blot analysis of EIF4B (arrow) using rabbit polyclonal EIF4B Antibody (S422) (RB13724). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF4B gene (Lane 2) (Origene Technologies).
	Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with EIF4B-S422, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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