Anti-EIF4E Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Accession Number	P06730
Gene Id	1977
Gene Symbols	EIF4E
Protein Name / Synonyms	EIF4E
Clonality	Monoclonal
Preparation	This EIF4E monoclonal antibody is generated from mouse immunized with EIF4E recombinant protein.
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

EIF4E recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

Specificity

Other Names

EIF4E; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein

NCBI Accession #

NP_001124150.1;NP_001124151.1;NP_001959.1

Other Accession #

NP_001959.1

Format

Type

Mouse Monoclonal Antibody (Mab)

Calculated Molecular Weight (Da)

25097

Recommended Dilutions

IF: 1:10-50; WB: 1:100-500

Fluorescent image of Hela cell stained with EIF4E Antibody(Cat# 102-10076/SG101020AF).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EIF4E primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). EIF4E immunoreactivity is localized to Cytoplasm significantly.

EIF4E Antibody (Cat# 102-10076) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EIF4E antibody detected the EIF4E protein (arrow).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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