Rabbit Anti-Human EIF4E Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P06730 |
| Gene Id | 1977 |
| Gene Symbols | EIF4E |
| Protein Name / Synonyms | EIF4E |
| Clonality | Polyclonal |
| Preparation | This EIF4E antibody is generated from rabbits immunized with a recombinant protein from human VGFR2. |
| Concentration (lot specific) | 0.360 mg/ml |
| Recommended Applications | FC, IHC-P, Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G.
Specificity
Other Names
EIF4E; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein
NCBI Accession #
NP_001124150.1;NP_001124151.1;NP_001959.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
25097
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | Western blot analysis of EIF4E Antibody (Cat# 102-14317) in HL-60 cell line lysates (35ug/lane). EIF4E (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human testis tissue reacted with EIF4E Antibody (Cat# 102-14317), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | EIF4E Antibody (Cat# 102-14317) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.