Anti-EIF4EBP1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q13541
Gene Id	1978
Gene Symbols	EIF4EBP1
Protein Name / Synonyms	EIF4EBP1, 4E-BP1, 4EBP1, BP-1, PHAS-I, eukaryotic translation initiation factor 4E-binding protein 1
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Research Area	mTOR Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW

12kDa

Observed MW

17kDa

Immunogen

A phospho specific peptide corresponding to residues surrounding T70 of human EIF4EBP1

Modification

Phospho T70

Storage

-20°C

Images

Western blot analysis of extracts of HeLa cells, using Phospho-EIF4EBP1-T70 antibody (144-61487) at 1:2000 dilution. HeLa cells were treated by Insulin (100 nM) at 37? for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit.
Exposure time: 3min.
Western blot - Phospho-EIF4EBP1-T70 pAb (144-61487)

Expiration:

12 months from the date of shipment when stored properly.

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