Rabbit Anti-Human EIF4EBP1 (Center) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q13541 |
| Gene Id | 1978 |
| Gene Symbols | EIF4EBP1 |
| Protein Name / Synonyms | EIF4EBP1 (Center) |
| Clonality | Polyclonal |
| Preparation | This EIF4EBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-61 amino acids from the Central region of human EIF4EBP1. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Research Area | mTOR Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq].
Specificity
Predicted Reactivity
Mouse, Rat, Bovine
Other Names
EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1
NCBI Accession #
NP_004086.1
Other Accession #
Q62622; Q60876; Q0P5A7; NP_004086.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
12580
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; FC: 1:10-50
 | Western blot analysis of EIF4EBP1 (arrow) using rabbit polyclonal EIF4EBP1 Antibody (Center) (Cat# 102-11664). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4EBP1 gene. |
 | EIF4EBP1 Antibody (Center) (Cat# 102-11664)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF4EBP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | EIF4EBP1 Antibody (Center) (Cat# 102-11664) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.
