Rabbit Anti-Human EIF4EBP1 (T69) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q13541 |
| Gene Id | 1978 |
| Gene Symbols | EIF4EBP1 |
| Protein Name / Synonyms | EIF4EBP1 (T69) |
| Clonality | Polyclonal |
| Preparation | This EIF4EBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-77 amino acids from human EIF4EBP1. |
| Concentration (lot specific) | 0.290 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Research Area | mTOR Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
EIF4EBP1 is one member of a family of translation repressor proteins. It directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Specificity
Predicted Reactivity
Bovine
Other Names
EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1
NCBI Accession #
NP_004086.1
Other Accession #
Q0P5A7; NP_004086
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
12580
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50
 | Western blot analysis of EIF4EBP1 (arrow) using rabbit polyclonal EIF4EBP1 Antibody (T69) (RB11407). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF4EBP1 gene (Lane 2) (Origene Technologies). |
 | Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with EIF4EBP1 Antibody (T69) (Cat# 102-14432), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.