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Anti-ELAVL1 Antibody
Catalog #: 144-61434
ELAVL1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q15717 |
| Gene Id | 1994 |
| Gene Symbols | ELAVL1 |
| Protein Name / Synonyms | ELAVL1, ELAV1, HUR, Hua, MelG, ELAV-like protein 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy.
Dilutions
WB 1:500 - 1:1000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
36kDa/38kDa
Observed MW
36kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ELAVL1 (NP_001410.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ELAVL1 antibody (144-61434) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.
Western blot analysis of extracts from normal (control) and ELAVL1 knockout (KO) 293T cells, using ELAVL1 antibody (144-61434) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human prostate using ELAVL1 antibody (144-61434) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using ELAVL1 antibody (144-61434) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using ELAVL1 antibody (144-61434) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using ELAVL1 antibody (144-61434) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using ELAVL1 antibody (144-61434) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.