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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q12926 |
| Gene Id | 1993 |
| Gene Symbols | ELAVL2 |
| Protein Name / Synonyms | ELAVL2, HEL-N1, HELN1, HUB, ELAV-like protein 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a neural-specific RNA-binding protein that is known to bind to several 3' UTRs, including its own and also that of FOS and ID. The encoded protein may recognize a GAAA motif in the RNA. Three transcript variants encoding two different isoforms have been found for this gene.
Dilutions
WB 1:1000 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
38kDa/39kDa
Observed MW
44kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ELAVL2 (NP_004423.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ELAVL2 antibody (144-05918) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using ELAVL2 antibody (144-05918) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using ELAVL2 antibody (144-05918) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using ELAVL2 antibody (144-05918) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.
