Anti-ELP2 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q6IA86
Gene Id	55250
Gene Symbols	ELP2
Protein Name / Synonyms	ELP2 (C-term)
Clonality	Polyclonal
Preparation	This ELP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 737-765 amino acids from the C-terminal region of human ELP2.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	IF, Western Blotting, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

ELP2 regulates the ligand-dependent activation of STAT3. The protein acts as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4.

Specificity

Other Names

ELP2; STATIP1; Elongator complex protein 2; SHINC-2; STAT3-interacting protein 1

NCBI Accession #

NP_001229804.1;NP_001229805.1;NP_001229806.1;NP_001229807.1;NP_001229808.1;NP_060725.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

92500

Recommended Dilutions

IF: 1:100; WB: 1:1000; FC: 1:10-50

	Fluorescent confocal image of SY5Y cells stained with ELP2 (C-term) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-15203 ELP2 (C-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). Note the highly specific localization of the ELP2 immunosignal mainly to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000134759).
	Western blot analysis of ELP2 antibody (C-term) (Cat# 102-15203) in NCI-H460 cell line lysates (35ug/lane). ELP2 (arrow) was detected using the purified Pab.
	Flow cytometric analysis of NCI-H292 cells using ELP2 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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