Anti-EML1 (C-term) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Gene Id	2009
Protein Name / Synonyms	EML1, C-term
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

ELP79 antibody, EMAP antibody, EMAPL antibody, FLJ45033 antibody, HuEMAP antibody, EML1 antibody, huEMAP-1 antibody, echinoderm microtubule-associated protein-like 1 antibody, EMAP-1 antibody, echinoderm microtubule associated protein like 1 antibody

Specificity

Species Reactivity

Human, Mouse

Format

Product Type

Primary antibody

Formulation

1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

90 kDa

Immunogen

Synthetic peptide corresponding to a region within amino acids 753 and 815 of EML1 (Uniprot ID# O00423)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed HeLa, using EML1(107-10198) antibody at 1:500 dilution.

Immunohistochemical analysis of paraffin-embedded RF48 xenograft, using EML1(107-10198) antibody at 1:500 dilution.

EML1 antibody [C3], C-term detects EML1 protein at cytosol on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
EML1 antibody [C3], C-term (107-10198) dilution: 1:500.

EML1 antibody [C3], C-term detects EML1 protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
EML1 antibody [C3], C-term (107-10198) dilution: 1:500.

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
EML1 antibody
107-10198 diluted at 1:500

Sample (30 ug of whole cell lysate)

A:NIH-3T3
7.5% SDS PAGE

107-10198 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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