Anti-EMX1 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q04741
Gene Id	2016
Gene Symbols	EMX1
Protein Name / Synonyms	EMX1 (C-term)
Clonality	Polyclonal
Preparation	This EMX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-257 amino acids from the C-terminal region of human EMX1.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IF, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

EMX1 is a transcription factor, which in cooperation with EMX2. It acts to generate the boundary between the roof and archipallium in the developing brain. It may function in combinations with OTX1/2 to specify cell fates in the developing central nervous system.

Specificity

Other Names

EMX1; Homeobox protein EMX1; Empty spiracles homolog 1; Empty spiracles-like protein 1

NCBI Accession #

NP_004088.2

Other Accession #

Q04742

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

28039

Recommended Dilutions

WB: 1:1000; FC: 1:10-50; IF: 1:10-50

	EMX1 Antibody (C-term) (Cat# 102-18099) western blot analysis in U251 and Neuro-2a cell line mouse cerebellum tissue lysates (35ug/lane).This demonstrates the EMX1 antibody detected the EMX1 protein (arrow).
	EMX1 Antibody (C-term) (Cat# 102-18099) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Fluorescent confocal image of Hela cell stained with EMX1 Antibody (C-term)(Cat# 102-18099).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EMX1 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). EMX1 immunoreactivity is localized to Nucleus significantly.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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