Anti-EN2 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P19622
Gene Id	2020
Gene Symbols	EN2
Protein Name / Synonyms	EN2 (C-term)
Clonality	Polyclonal
Preparation	This EN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-271 amino acids from the C-terminal region of human EN2.
Concentration (lot specific)	0.450 mg/ml
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

EN2 is thought to have a role in controlling development. In Drosophila, the protein plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. This protein has been implicated in the control of pattern formation during development of the central nervous system.

Specificity

Predicted Reactivity

Zebrafish, Chicken, Xenopus

Other Names

EN2; Homeobox protein engrailed-2

NCBI Accession #

NP_001418.2

Other Accession #

P09066; Q05917; P52730; P52729; P09015

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

34211

Recommended Dilutions

WB: 1:1000; IF: 1:10-50

Western blot analysis of EN2 antibody (C-term) (Cat# 102-17362) in 293 cell line lysates and mouse stomach and lung tissue lysates (35ug/lane). EN2 (arrow) was detected using the purified Pab.

Fluorescent confocal image of Hela cell stained with EN2 Antibody (C-term)(Cat# 102-17362). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EN2 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). EN2 immunoreactivity is localized to nucleus significantly and Cytoplasm weakly.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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