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Anti-ENO1 Antibody
Catalog #: 107-10290
ENO1 antibody [N3C3], 1 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 2023 |
| Protein Name / Synonyms | ENO1 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
ENO1L1 antibody, MPB1 antibody, NNE antibody, PPH antibody, ENO1 antibody, non-neural enolase antibody, 2-phospho-D-glycerate hydro-lyase antibody, alpha-enolase, c-myc promoter-binding protein-1 antibody, alpha enolase like 1 antibody, tau-crystallin antibody, alpha-enolase antibody, MYC promoter-binding protein 1 antibody, phosphopyruvate hydratase antibody, plasminogen-binding protein antibody, enolase-alpha antibody, "enolase 1, (alpha) antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
47 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 199 and 434 of ENO1 (Uniprot ID# P06733)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded gastric tissue, using ENO1(107-10290) antibody at 1:100 dilution.
Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
107-10290 diluted at 1:1000
A: Mouse brain
10% SDS PAGE
107-10290 diluted at 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using enolase 1(107-10290) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate)
A: A431
B: H1299
10% SDS PAGE
107-10290 diluted at 1:3000
A: A431
B: H1299
10% SDS PAGE
107-10290 diluted at 1:3000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.