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Anti-ENOB (Center) Antibody
Catalog #: 102-15204
Rabbit Anti-Human ENOB (Center) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P13929 |
| Gene Id | 2027 |
| Gene Symbols | ENO3 |
| Protein Name / Synonyms | ENOB (Center) |
| Clonality | Polyclonal |
| Preparation | This ENOB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237-264 amino acids from the Central region of human ENOB. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
ENOB is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in its gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme.
Specificity
Predicted Reactivity
Mouse, Rat, Bovine, Pig
Other Names
ENO3; Beta-enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 3; Muscle-specific enolase; Skeletal muscle enolase
NCBI Accession #
NP_001180432.1;NP_001967.3;NP_443739.3
Other Accession #
P15429; Q1KYT0; P21550; Q3ZC09
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
46987
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | ENOB Antibody (Center) (Cat# 102-15204) western blot analysis in RD cell line and mouse skeletal muscle,rat skeletal muscle lysates (35ug/lane).This demonstrates the ENOB antibody detected the ENOB protein (arrow). |
 | Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ENOB Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | ENOB Antibody (Center) (Cat# 102-15204) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.