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Anti-eNos (S1177) Antibody
Catalog #: 102-11274
Rabbit Anti-Human eNos (S1177) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P29474 |
| Gene Id | 4846 |
| Gene Symbols | NOS3 |
| Protein Name / Synonyms | eNos (S1177) |
| Clonality | Polyclonal |
| Preparation | This eNos antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1156-1183 amino acids from human eNos. |
| Concentration (lot specific) | 0.250 mg/ml |
| Recommended Applications | Western Blotting, IF, FC |
| Research Area | Angiogenesis, Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene.
Specificity
Predicted Reactivity
Mouse, Rat, Bovine, Pig
Other Names
NOS3; Nitric oxide synthase, endothelial; Constitutive NOS; EC-NOS; Endothelial NOS; NOS type III
NCBI Accession #
NP_000594.2;NP_001153581.1;NP_001153582.1;NP_001153583.1
Other Accession #
Q62600; Q28969; P70313; P29473; NP_001153582.1; NP_000594.2; NP_001153581.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
133289
Recommended Dilutions
WB: 1:1000; FC: 1:10-50; IF: 1:10-50
 | eNos Antibody (S1177) (Cat# 102-11274) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the eNos antibody detected the eNos protein (arrow). |
 | eNos Antibody (S1177) (Cat# 102-11274) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Confocal immunofluorescent analysis of eNos Antibody (S1177)(Cat# 102-11274) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.